Proteomics Laboratory
Laboratory Home Overview:

Proteomics Laboratory is involved in developing approaches for determination effects of toxicants and identification of novel protein biomarkers of toxicity, and mapping the serum/plasma and other biological proteomes which are indicative of the toxicity and could help in understanding the complex mechanistic pathways. Proteomic approach like two dimensional gel electrophoresis is being applied in vivo, in vitro as well as in cancer patients, to find out a potential biomarker of clinical utility. In addition, considerable emphasis is being laid down on the use of dietary phytochemicals for chemoprevention due to their relative non-toxic effects. The different bio-molecules involved in cell signaling transduction pathways are targeted to understand the mechanisms that activate cell death signal and induce apoptosis in cancer cells resulting in inhibition of cancer development or progression, at both gene and protein level. To achieve the above objectives we have been employing sensitive techniques like western blot analysis, immunohistochemistry, flow cytometry and reverse transcription polymerase chain reaction 

Planned activities: 

  • Toxicoproteomics: Toxicoproteomics will be applied to develop biomarkers for assessing toxic responses from pesticides, metals and pollutants/contaminants through proteomic approaches. Comparative proteome strategy utilized here will shed light on molecular mechanism of toxicity. 

  • Plasma proteomics: Plasma proteomics will be employed to investigate the plasma profiling of cancer patients with major emphasis on oral, prostate and breast cancer. The plasma proteomic profiling of the northern Indian population will also be undertaken for developing novel biomarkers.

  Major activities:
 
  • To identify and validate potential protein biomarkers of clinical utility in cancer by using proteomics approaches.
  Highlights of Research:  

  • Proteomic approaches are being applied for developing new biomarkers /toxicity signatures and mapping the serum/plasma and other biological proteomes which are indicative of the toxicity which could help in understanding the complex mechanistic pathways.

  • Role of different dietary agents in cancer chemoprevention is being studied by targeting different proteins involved in cell signaling transduction pathways to investigate the mechanisms that activate cell death signal and induce apoptosis in precancerous or cancer cells resulting in inhibition of cancer development or progression.

  Major Facilities available:

  • Two dimensional gel electrophoresis

  • Matrix assisted laser desorption ionization time of flight (MALDI-TOF)

  • Western blotting

Staff:
Dr. Yogeshwer Shukla, Scientist F & Head
Mr. S.H.N. Naqvi, Technical Assistant

Project Staff:
Dr. Madhulika Singh, Research Associate
Ms. Preeti Roy, Research Associate
Dr. Alok Yadav, Research Associate
Mr. Sahdeo Prasad, SRF
Ms. Shilpa Tyagi, JRF
Mr. H.S. Maan, JRF
Ms. Nidhi Nigam, Project Assistant
Ms. Jasmine George, Project Assistant
Ms. Kulpreet Bhui, Project Assistant
Mrs. Richa Singh, Project Assistant

 

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